Biolegend, 408004, Biotin anti-Allophycocyanin (APC) Antibody, 100μg

The APC003 antibody reacts with both allophycocyanin (APC) alone and APC conjugated with antibody or streptavidin, as well as APC tandem dyes such as APC/Cyanine5.5 and APC/Cyanine7. When APC003 antibody is used in a three-step staining procedure or separation of APC-antibody labelled cells, it neither quenches APC fluorescence nor reacts with tested mouse and human cells. The APC003 antibody can be used for separation of APC-antibody labeled cells or as a secondary step to amplify APC conjugated primary antibody signals (i.e. biotinylated APC003 as secondary step, followed by Streptavidin-APC).
100μg
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: allophycocyanin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. Use at concentrations comparable to those of the primary antibody of interest. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Safi F 2023. STAR Protocols. 4(1):101965. PubMed Illingworth J, et al. 2013. J Immunol. 190:1038. PubMed Marangoni F, et al. 2021. Cell. . PubMed Coppieters K, et al. 2012. J Exp Med. 209:51:00. PubMed Matsushita H, et al. 2015. Cancer Immunol Res. 3:26. PubMed
RRID: AB_345359 (BioLegend Cat. No. 408003) AB_345360 (BioLegend Cat. No. 408004)
Gene ID: NA
UniProt: View information about Allophycocyanin on UniProt.org
Clone: APC003
Regulatory Status: RUO
Isotype: Mouse IgG1, κ
Q: How many biotin molecules are per antibody structure?
A: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.