Biolegend, 637853, Ultra-LEAF™ Purified anti-human TNFSF18 (GITRL) Antibody, 100μg

TNFSF18 is a type II transmembrane glycoprotein that belongs to the TNF superfamily. This protein is mainly expressed on antigen presenting cells (APC) including macrophages, B cells, dendritic cells (DCs), and endothelial cells. Its expression increases upon the stimulation of proinflammatory stimuli. The receptor of TNFSF18 is the glucocorticoid-induced TNFR-related protein (GITR), which is a member of the TNFR superfamily. Engagement of TNFSF18 and GITR leads to T cell activation, proliferation, and cytokine production. It also inhibits the suppressive function of T regulatory cells and induces macrophage activation. GITR-TNFSF18 interaction plays an essential role in the regulation of the immune response to self-antigens and pathogens. In addition, this receptor-ligand interaction has been shown to modulate anti-tumor immunity.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human TNFSF18 (GITRL)
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 0.15 µg/mL human TNFSF18 (GITRL) to 2 µg/mL immobilized recombinant human GITR (TNFRSF18) Fc chimera. ND50 range: 0.12 – 1 mg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3106203 (BioLegend Cat. No. 637853) AB_3106203 (BioLegend Cat. No. 637854)
Structure: Monomer
Distribution: Macrophages, B cells, dendritic cells (DCs), and endothelial cells
Function: TNFSF18 plays roles in regulating T cell activation and natural killer cell anti-tumor activity. It is induced by inflammatory processes.
Interaction: T cells
Ligand/Receptor: GITR (TNFRSF18)
Cell Type: Embryonic Stem Cells
Biology Area: Cell Proliferation and Viability, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Yu KY, et al. 2003. Biochem Biophys Res Commun. 310:433-8. Kim JD, et al. 2003. Genes Immun. 4:564-9. Azuma M. 2010. Crit Rev Immunol. 30:547-57. Hanabuchi S, et al. 2006. Blood. 107:3617-23. Muriglan SJ, et al. 2004. J Exp Med. 200:149-57. Shevach EM and Stephens GL. 2006. Nat Rev Immunol. 6:613-8. Baltz K, et al. 2007. FASEB J. 21:2442-54. Placke T, et al. 2010. Clin Dev Immunol. 2010:239083.
Gene ID: 8995
UniProt: View information about TNFSF18 on UniProt.org
Clone: W21092F
Regulatory Status: RUO
Other Names: TL6, AITRL, GITRL, GITR Ligand, Tumor necrosis factor superfamily, Member 18
Isotype: Rat IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.