Biolegend, 640354, Ultra-LEAF™ Purified anti-mouse CD112R (PVRIG) Antibody, 1mg

CD112R gene encodes a single transmembrane protein consisting of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain which contains two tyrosine residues, one within an ITIM-like motif. CD112R was initially named PVRIG for the homology observed between its second exon and the variable immunoglobulin domain of the polio virus receptor (PVR/CD155) and polio virus receptor-like (PVRL) genes. CD112R is a cell surface receptor for CD112/Nectin-2 and may act as a coinhibitory receptor that suppresses T cell receptor-mediated signals. Following interaction with CD112, CD112R inhibits T cell proliferation and the disruption of interaction between CD112R and CD112 enhances T cell response which indicated that CD112R may be a novel checkpoint for human T cells. CD112R competes with CD226 for CD112 binding. It has been demonstrated that blockade of CD112R enhances Trastuzumab, first-line drug to treat breast cancer with high Her2 expression, triggered antitumor response by human NK cells.
1mg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant Mouse CD112R
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Block - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by blocking the binding of 700 ng/mL Biotinylated Recombinant Mouse CD112-Fc Chimera to 1 μg/mL immobilized Recombinant Mouse CD112R-Fc Chimera. ND50 range: 13 – 130 ng/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3717154 (BioLegend Cat. No. 640353) AB_3717154 (BioLegend Cat. No. 640354)
Structure: Homodimer
Distribution: Preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells.
Function: Cell surface receptor for CD112 that may act as a coinhibitory receptor that suppresses T cell receptor-mediated signals.
Interaction: T cells, NK cells
Ligand/Receptor: CD112/Nectin-2
Cell Type: Leukocytes, Lymphocytes, NK cells, T cells
Biology Area: Adaptive Immunity, Immuno-Oncology, Immunology, Inhibitory Molecules
Molecular Family: Immune Checkpoint Receptors
Antigen References: Xu F, et al. 2017. Cancer Immunol Immunother. 66:1367. Zhu Y, et al. 2016. J Exp Med. 213:167. Zeng T, et al. 2021. J Exp Clin Cancer Res. 40:285.
Gene ID: 620147
UniProt: View information about CD112R on UniProt.org
Clone: W19184B
Regulatory Status: RUO
Other Names: PVRIG, PVR Related Immunoglobulin Domain Containing, C7orf15, CD112R, CD112 Receptor
Isotype: Rat IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.