Biolegend, 648004, Alexa Fluor® 488 anti-Hsp70 Antibody, 100tests

Hsp70 is a member of the Hsp family of highly conserved proteins that play a role in a number of important functions. Their synthesis is increased upon environmental stress. They function by acting as molecular chaperones, facilitating protein refolding and preventing protein aggregation. It has been reported that extracellular Hsp70 also exhibits immunomodulatory effects on innate and acquired immunity.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Hsp70 protein from HeLa cells.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Product Citations: Tsai KC, et al. 2023. Mar Drugs. 21:. PubMed Gilazieva Z, et al. 2022. Curr Issues Mol Biol. 44:5363. PubMed Chulpanova DS, et al. 2023. Curr Issues Mol Biol. 45:571. PubMed Salybekov AA, et al. 2021. Front Cardiovasc Med. 8:754254. PubMed Mouratidis PXE, et al. 2021. Int J Hyperthermia. 38:1443. PubMed Pascual-Pasto G, et al. 2022. J Immunother Cancer. 10:. PubMed Kırbaş OK, et al. 2019. Sci Rep. 9:19159. PubMed
RRID: AB_2119539 (BioLegend Cat. No. 648003) AB_2119537 (BioLegend Cat. No. 648004)
Structure: Hsp proteins are the most conserved proteins present in both prokaryotes and eukaryotes. Human HSp70 contains multiple isoforms. Hsp70s consisit of three structural domains, a 44Kd amino-terminal ATPase domain (NBD), followed by an 18 Kd substrate
Distribution: Ubiquitous, cytoplasmic and nuclear
Function: Molecular chaperrones assisting in the folding of proteins and preventing protein aggregation
Biology Area: Cell Biology, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance
Antigen References: 1. Sharma D, et al. 2009. Protein Peptide Lett. 16:571. 2. Johnson JD, et al. 2006. J. Leuk. Biol. 79:425. 3. Bukau B, et al. 1998. Cell 92:351.
Gene ID: 3308
UniProt: View information about Hsp70 on UniProt.org
Clone: W27
Regulatory Status: RUO
Other Names: Heat shock protein 70
Isotype: Mouse IgG2a, λ