Biolegend, 648851, Purified anti-CAV1 (Caveolin-1), 100μg

Caveolin-1 (CAV1) is a critical structural protein present in caveolae, small invaginations found in the plasma membrane of many cell types. Caveolin-1 helps in the formation and stability of caveolae, acting as a scaffolding protein. It plays a key role in organizing and regulating signaling molecules, influencing various cellular processes such as endocytosis, cholesterol homeostasis, and signal transduction. Additionally, it is involved in modulating cell proliferation, apoptosis, and mechanotransduction. Dysregulation of Caveolin-1 has been linked to several diseases including cancer, cardiovascular disorders, and fibrosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein corresponding to the C terminus of human Caveolin-1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following:-Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100-Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol-100% ice-cold methanol onlyFor use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with any of the following:-Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803)-True-Phos™ Perm Buffer (Cat. No. 425401)-True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401)
RRID: AB_3720576 (BioLegend Cat. No. 648850) AB_3720576 (BioLegend Cat. No. 648851)
Structure: Caveolin-1 is a 178 amino acid protein with a predicted molecular weight of 20.5 kD.
Distribution: Mostly cytoplasmic but can be localized to cell membrane.
Function: Structural protein
Interaction: CAV2, proteins containing caveolin-binding motifs such as Src family kinases, heterotrimeric G-proteins
Biology Area: Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Protein Trafficking and Clearance, Signal Transduction
Molecular Family: Scaffold Proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: Wend P, et al. 2013. EMBO J. Welm AL, et al. 2005. Proc Natl Acad Sci USA. 102:4324-9. Hu Y, et al. 2001. Nature. 410:710-714. Yuspa SH, et al. 1989. J Cell Biol. 109:1207-1217. Roop DR, et al. 1984. J Biol Chem. 259:8037-8040.
Gene ID: 857
UniProt: View information about CAV1 on UniProt.org
Clone: W23150D
Regulatory Status: RUO
Other Names: Caveolin-1 (CAV1), Vesicular Integral Membrane Protein 21 (VIP21)
Isotype: Rat IgG2a, λ