CST, 10131SF, CSF-1R/M-CSF-R (D9A9Y) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying CSF1R. Validated for ELISA+. Highly specific and rigorously validated in-house, CSF-1R/M-CSF-R (D9A9Y) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #10131) is ready to ship. Product Usage Information This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CSF-1R/M-CSF-R (D9A9Y) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CSF-1R/M-CSF-R protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant CSF-1R/M-CSF-R protein. Background Macrophage-colony stimulating factor (M-CSF, CSF-1) receptor is an integral membrane tyrosine kinase encoded by the proto-oncogene. M-CSF receptor is expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage (1-3). Binding of M-CSF to its receptor induces receptor dimerization, activation, and autophosphorylation of cytoplasmic tyrosine residues used as docking sites for SH2-containing signaling proteins (4). There are at least five major tyrosine autophosphorylation sites. Tyr723 (Tyr721 in mouse) is located in the kinase insert (KI) region. Phosphorylated Tyr723 binds the p85 subunit of PI3 kinase as well as PLCγ2 (5). Phosphorylation of Tyr809 provides a docking site for Shc (5). Overactivation of this receptor can lead to a malignant phenotype in various cell systems (6). The activated M-CSF receptor has been shown to be a predictor of poor outcome in advanced epithelial ovarian carcinoma (7) and breast cancer (8). Alternate Names BANDDOS; C-FMS; CD115; CD115 antigen; colony stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; CSF1R; CSFR; FIM2; FMS; FMS proto-oncogene; HDLS; M-CSF-R; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; Proto-oncogene c-Fms Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG