CST, 10371T, Glycogen Synthase 1 (GYS1) (F4L8X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Glycogen Synthase 1. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, Glycogen Synthase 1 (GYS1) (F4L8X) Rabbit Monoclonal Antibody (CST #10371) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Glycogen Synthase 1 (GYS1) (F4L8X) Rabbit Monoclonal Antibody recognizes endogenous levels of total GYS1 protein. This antibody does not cross-react with GYS2 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GYS1 protein. Background Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver (1). Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals: muscle isoform (glycogen synthase 1, GYS1) and liver isoform (glycogen synthase 2, GYS2), respectively (1). Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649, and Ser653), inhibiting its activity (2,3). Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth (1). Alternate Names Glycogen [starch] synthase, muscle; glycogen synthase 1; glycogen synthase 1 (muscle); GSY; GYS; GYS1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 84 Source/Isotype: Rabbit IgG