CST, 10630SF, GATA-3 (D13C9) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying GATA3. Validated for WB,IHC,IF,F. Highly specific and rigorously validated in-house, GATA-3 (D13C9) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #10630) is ready to ship. Product Usage Information This product is the carrier-free version of product #5852. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 5852 . Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity GATA-3 (D13C9) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total GATA-3 protein. This antibody is not recommended for immunofluorescence analysis of frozen mouse tissues due to non-specific staining. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr63 of human GATA-3 protein. Background GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA-binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3). GATA-3 is a critical regulator of development of various systems in both mouse and human (4). GATA-3 mouse embryos die between E11 and E12 due to growth retardation and deformities in the brain and spinal cord (5). The function of GATA-3 has been extensively studied in T cell development and has recently been shown to be a downstream target of Notch in Notch-mediated differentiation of TH2 cells (6,7). It is expressed in both hematopoietic and non-hematopoietic tissues, including the kidney, skin, mammary gland, and central nervous system (8-10). Decreased expression of GATA-3 in luminal breast cancer is associated with poor clinical outcome. GATA-3 expression level may therefore be a promising prognostic biomarker (11). Haploinsufficiency of GATA-3 results in Barakat syndrome in humans, a condition characterized by sensorineural deafness and renal dysplasia (12). Alternate Names GATA binding protein 3; GATA-binding factor 3; GATA3; HDR; HDRS; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3 Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG