CST, 11816S, DEPTOR/DEPDC6 (D9F5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Deptor. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, DEPTOR/DEPDC6 (D9F5) Rabbit Monoclonal Antibody (CST #11816) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunohistochemistry (Paraffin): 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 25285 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin) Specificity / Sensitivity DEPTOR/DEPDC6 (D9F5) Rabbit Monoclonal Antibody recognizes endogenous levels of total DEPTOR/DEPDC6 protein. Species Reactivity: Human, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu113 of human DEPTOR/DEPDC6 protein. Background DEPTOR/DEPDC6 is a component of both mTORC1 and mTORC2 complexes (1). It interacts with mTOR and inhibits mTORC1 and mTORC2 kinase activities (1). mTOR, along with casein kinase I, phosphorylates DEPTOR/DEPDC6 in the presence of growth signals, which leads to the degradation of DEPTOR/DEPDC6 (2). Research studies have shown that transgenic mice overexpressing DEPTOR/DEPDC6 have more white adipose tissue, and DEPTOR/DEPDC6 expression levels increase in the white adipose tissue of obese humans (3). Furthermore, the expression of DEPTOR/DEPDC6 is induced during mouse adipocyte differentiation (3). Together these findings suggest that DEPTOR/DEPDC6 regulates adipogenesis (3). Alternate Names DEP domain containing 6; DEP domain containing mTOR interacting protein; DEP domain-containing mTOR-interacting protein; DEP domain-containing protein 6; DEP.6; DEPD6; DEPDC6; Deptor; DKFZp564B1778; DPTOR; FLJ12341; FLJ12428; FLJ13854 Specification REACTIVITY: H R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG