CST, 11831S, WBP2 Antibody

Polyclonal Antibody for studying WBP2. Validated for Western Blotting. Highly specific and rigorously validated in-house, WBP2 Antibody (CST #11831) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity WBP2 Antibody recognizes endogenous levels of total WBP2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn239 of human WBP2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background WW-domain binding protein 2 (WBP2) is an adaptor protein first identified in a screen for proteins that interact with YAP1 (1). WBP2 was subsequently shown to bind other WW domain-containing proteins, including TAZ and NEDD4-like ubiquitin-protein ligases (2-5). There is strong evidence for a conserved functional role for WBP2 in the Hippo kinase tumor suppressor pathway. WBP2 interaction is required for the oncogenic properties of TAZ in breast cancer cells (4) and the growth-promoting properties of the YAP homolog Yorkie in (6). studies have also suggested that WBP2 may act as a transcriptional co-activator of both estrogen and progesterone receptors (7); this interaction was shown to be dependent upon tyrosine phosphorylation of WBP2 by c-Src and c-Yes kinases (8). Alternate Names DFNB107; GRAMD6; MGC18269; WBP-2; WBP2; WW domain binding protein 2; WW domain-binding protein 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 32 SOURCE: Rabbit