CST, 11864T, PSMA2 (D3A4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PSMA2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PSMA2 (D3A4) Rabbit Monoclonal Antibody (CST #11864) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PSMA2 (D3A4) Rabbit Monoclonal Antibody recognizes endogenous levels of total PSMA2 protein. This antibody does not cross-react with other α subunits of the 20S proteasome. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe173 of human PSMA2 protein. Background The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of α subunits (called PSMAs), and the two inner layers are identical rings composed of β subunits. While the catalytic sites are located on the β rings (1-3), the α subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different α and ten different β proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades polyubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9). Alternate Names HC3; Macropain subunit C3; MU; Multicatalytic endopeptidase complex subunit C3; PMSA2; proteasome (prosome, macropain) subunit, alpha type, 2; proteasome 20S subunit alpha 2; Proteasome component C3; proteasome subunit alpha 2; Proteasome subunit alpha type-2; proteasome subunit HC3; PSA2; PSC2; PSC3; PSMA2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 25 Source/Isotype: Rabbit IgG