CST, 11911S, NFI-C Antibody

Polyclonal Antibody for studying NFI-C. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, NFI-C Antibody (CST #11911) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity NFI-C Antibody recognizes endogenous levels of total NFI-C protein. This antibody also recognizes overexpressed NFI-B protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala263 of human NFI-C protein. Antibodies are purified by protein A and peptide affinity chromatography. Background NFI-C belongs to the nuclear factor I (NFI) family of site-specific transcription factors that regulate viral DNA replication and expression of various genes (1,2). The NFI family is composed of four members in vertebrates: NFI-A, NFI-B, NFI-C, and NFI-X, all of which are critical in the development of multiple organ systems in mice and humans (3). NFI-C is expressed in various tissues and regulates TGF-β dependent tooth development and hair follicle cycling (3-5). Research studies have shown that NFI-C directly represses FoxF1 transcription and suppresses the motility and invasiveness of breast cancer cells (6). Alternate Names CCAAT-binding transcription factor; CCAAT-box-binding transcription factor; CTF; CTF5; MGC20153; NF-I; NF-I/C; NF1-C; NFI; NFI-C; NFIC; Nuclear factor 1 C-type; Nuclear factor 1/C; nuclear factor I C; Nuclear factor I/C; nuclear factor I/C (CCAAT-binding transcription factor); TGGCA-binding protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 55-65 SOURCE: Rabbit