CST, 11989T, Phospho-p90RSK (Ser380) (D3H11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying RSK2 (Ser386) phosphate/RSK3 (Ser377) phosphate/RSK1 (Ser380) phosphate. Validated for Western Blotting,Immunohistochemistry (Paraffin),Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-p90RSK (Ser380) (D3H11) Rabbit Monoclonal Antibody (CST #11989) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:300 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 93317 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-p90RSK (Ser380) (D3H11) Rabbit Monoclonal Antibody recognizes endogenous levels of p90RSK1 protein when phosphorylated at Ser380. This antibody also detects p90RSK2 phosphorylated at Ser386 and p90RSK3 phosphorylated at Ser377. Species Reactivity: Human, Mouse, Rat, Monkey, Mink Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser377 of human p90RSK3 protein. Background The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed Ser/Thr kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, autophosphorylation, and phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3). Upon mitogenic stimulation, p44/42 Erk1/2 and Erk5 MAP kinases cooperatively phosphorylate p90RSK at Thr573 (RSK1 numbering) located within the C-terminal kinase domain and at Thr359/Ser363 in the linker region between the two kinase domains (3). Phosphorylation at Thr573 within the activation loop of the p90RSK C-terminal kinase domain promotes activation and phosphorylation at Ser380 within the a hydrophobic stretch of the linker region (4,5). When phosphorylated, Ser380 acts as a docking site for the constitutively active Ser/Thr kinase PDK1, which in turn phosphorylates p90RSK at Ser221 within the N-terminal kinase domain activation loop, resulting in full enzymatic activation of p90RSK (6). Antibodies against these phosphorylation sites are useful for understanding the kinetics and regulation of p90RSK activation.For more information regarding the phospho-regulatory sites within each p90RSK isoform, including more information regarding the seminal studies demonstrating the complex phosphorylation cascades involved, please see the references herein and PhosphoSitePlus (www.phosphosite.org). Alternate Names 90 kDa ribosomal protein S6 kinase 1; 90 kDa ribosomal protein S6 kinase 2; 90 kDa ribosomal protein S6 kinase 3; CLS; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); epididymis secretory sperm binding protein; HU-1; HU-2; HU-3; Insulin-stimulated protein kinase 1; ISPK-1; ISPK1; KS6A1; KS6A2; KS6A3; MAP kinase-activated protein kinase 1a; MAP kinase-activated protein kinase 1b; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1a; MAPK-activated protein kinase 1b; MAPK-activated protein kinase 1c; MAPKAP kinase 1a; MAPKAP kinase 1b; MAPKAP kinase 1c; MAPKAPK-1a; MAPKAPK-1b; MAPKAPK-1c; MAPKAPK1; MAPKAPK1A; MAPKAPK1B; MAPKAPK1C; mental retardation, X-linked 19; mitogen-activated protein kinase-activated protein kinase 1C; MRX19; p90-RSK 1; p90-RSK 2; p90-RSK 3; p90-RSK2; p90-RSK3; p90RSK; p90RSK1; p90RSK2; p90RSK3; p90S6K; pp90RSK2; pp90RSK3; ribosomal protein S6 kinase A1; ribosomal protein S6 kinase A2; ribosomal protein S6 kinase A3; ribosomal protein S6 kinase alpha 1; ribosomal protein S6 kinase alpha 2; Ribosomal protein S6 kinase alpha-1; Ribosomal protein S6 kinase alpha-2; Ribosomal protein S6 kinase alpha-3; ribosomal protein S6 kinase, 90kDa, polypeptide 1; ribosomal protein S6 kinase, 90kDa, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 3; Ribosomal S6 kinase 1; Ribosomal S6 kinase 2; Ribosomal S6 kinase 3; RPS6KA1; RPS6KA2; RPS6KA3; RSK; RSK-1; RSK-2; RSK-3; RSK1; RSK2; RSK3; S6K-alpha; S6K-alpha 1; S6K-alpha-1; S6K-alpha-2; S6K-alpha-3; S6K-alpha2; S6K-alpha3 Specification REACTIVITY: H M R Mk Mi SENSITIVITY: Endogenous MW (kDa): 90 Source/Isotype: Rabbit IgG