CST, 12048T, EREG (D4O5I) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying EREG. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, EREG (D4O5I) Rabbit Monoclonal Antibody (CST #12048) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity EREG (D4O5I) Rabbit Monoclonal Antibody recognizes endogenous levels of proepiregulin and the C-terminal propeptide of the EREG protein. This antibody does not recognize the mature form of EREG. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu155 of human EREG protein. Background Epiregulin (EREG) belongs to the epidermal growth factor (EGF) family and is a ligand for the EGF receptor (EGFR) and ErbB4 (1-3). The binding of EREG to homodimers, as well as heterodimers containing EGFR or ErbB4, leads to receptor activation and downstream signaling to promote cell growth and proliferation (4-6). In normal human tissue, moderate levels of EREG are expressed in the placenta and peripheral blood macrophages. Research studies have shown that EREG is expressed at high levels in numerous cancer cell lines, and EREG expression is correlated with primary cancer aggresiveness/metastases (7-11). In addition to its involvement in tumorigenesis, a variant of EREG has also been shown to be associated with tuberculosis susceptibility (12). EREG is synthesized as a ~30 kDa glycosylated membrane bound proepiregulin form (19 kDa when not glycosylated) and through subsequent proteolytic cleavage is processed to a 17 kDa C-terminal propeptide, and the 6kDa mature form of epiregulin (13). Alternate Names Ep; Epiregulin; EPR; ER; EREG; Proepiregulin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 17,19, 30 Source/Isotype: Rabbit IgG