CST, 12163T, IL-10 (D13A11) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL10. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-10 (D13A11) Rabbit Monoclonal Antibody (CST #12163) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IL-10 (D13A11) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-10 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant human IL-10 protein. Background Interleukin-10 (IL-10) is an anti-inflammatory cytokine that is produced by T cells, NK cells, and macrophages (1,2). IL-10 initiates signal transduction by binding to a cell surface receptor complex consisting of IL-10 RI and IL-10 RII (1), leading to the activation of Jak1 and Tyk2 and phosphorylation of Stat3 (1,3). The anti-inflammatory activity of IL-10 is due to its ability to block signaling through other cytokine receptors, notably IFN-γ receptor, by upregulating expression of SOCS1 (1,3). In addition, IL-10 promotes T cell tolerance by inhibiting tyrosine phosphorylation of CD28 (4,5). IL-10 is an important negative regulator of the immune response, which allows for maintenance of pregnancy (1). In contrast, increased IL-10 levels contribute to persistent infections (6). Alternate Names CSIF; Cytokine synthesis inhibitory factor; GVHDS; IL-10; IL10; IL10A; interleukin 10; Interleukin-10; MGC126450; MGC126451; T-cell growth inhibitory factor; TGIF Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 17 Source/Isotype: Rabbit IgG