CST, 12270S, Synaptophysin (7H12) Mouse Monoclonal Antibody

Monoclonal Antibody for studying SYP. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Synaptophysin (7H12) Mouse Monoclonal Antibody (CST #12270) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 36451 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Synaptophysin (7H12) Mouse Monoclonal Antibody recognizes endogenous levels of total synaptophysin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human synaptophysin protein. Background Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4). Alternate Names Major synaptic vesicle protein p38; MRX96; MRXSYP; Synaptophysin; SYP; SYPH Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Mouse IgG1