CST, 12310S, NKX2.2 Antibody

Polyclonal Antibody for studying NKX2-2. Validated for Western Blotting. Highly specific and rigorously validated in-house, NKX2.2 Antibody (CST #12310) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NKX2.2 Antibody recognizes transfected levels of total NKX2.2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala92 of human NKX2.2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The NKX family of homeobox genes are known to act as intermediaries in the neural response to Sonic hedgehog signaling during central nervous system (CNS) development (1). NKX2.2 is a member of this family of transcription factors and is necessary for neuroendocrine differentiation in the CNS and pancreas (2,3). NKX2.2 mutant mice die shortly after birth due to incomplete differentiation of insulin-producing pancreatic β cells and defects in ventral neural patterning (2,3). According to the research literature, expression of NKX2.2 has also been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors (4). Alternate Names Homeobox protein NK-2 homolog B; Homeobox protein Nkx-2.2; NK-2 homolog B; NK2 homeobox 2; NK2 transcription factor related, locus 2; NK2 transcription factor-like protein B; NKX2-2; NKX2.2; NKX22; NKX2B Specification REACTIVITY: H SENSITIVITY: Transfected Only MW (kDa): 40 SOURCE: Rabbit