CST, 12402T, PI3 Kinase Class II alpha (D3Q5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PIK3C2A. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, PI3 Kinase Class II alpha (D3Q5B) Rabbit Monoclonal Antibody (CST #12402) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity PI3 Kinase Class II alpha (D3Q5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total PI3K class II α protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly717 of human PI3K class II α protein. Background Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II α is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II α regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II α has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3). Alternate Names C2-containing phosphatidylinositol kinase; CPK; DKFZp686L193; MGC142218; OCSKD; P3C2A; Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha; Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha; phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha; Phosphoinositide 3-kinase-C2-alpha; phosphoinositide-3-kinase, class 2, alpha polypeptide; PI3-K-C2(ALPHA); PI3-K-C2A; PI3K-C2-alpha; PI3K-C2alpha; PIK3C2A; PtdIns-3-kinase C2 subunit alpha Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 180 Source/Isotype: Rabbit IgG