CST, 12511S, FGF Receptor 1 (D8E4) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying FGFR1. Validated for Western Blotting. Highly specific and rigorously validated in-house, FGF Receptor 1 (D8E4) Rabbit Monoclonal Antibody (Biotinylated) (CST #12511) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity FGF Receptor 1 (D8E4) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total FGF receptor 1 protein. This antibody does not cross-react with other FGF receptor family members. Species Reactivity: Human, Mouse, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human FGF receptor 1 protein. Background Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand-binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components, such as Crk and PLCγ (4,5). Alternate Names Basic fibroblast growth factor receptor 1; bFGF-R-1; BFGFR; CD331; CEK; ECCL; FGFBR; FGFR-1; FGFR1; FGFR1/PLAG1 fusion; Fibroblast growth factor receptor 1; FLG; FLJ99988; FLT-2; FLT2; Fms-like tyrosine kinase 2; fms-related tyrosine kinase 2; HBGFR; heparin-binding growth factor receptor; HH2; HRTFDS; hydroxyaryl-protein kinase; KAL2; N-SAM; OGD; Proto-oncogene c-Fgr; soluble FGFR1 variant 1; soluble FGFR1 variant 2 Specification REACTIVITY: H M Mk SENSITIVITY: Endogenous MW (kDa): 92, 120, 145 Source/Isotype: Rabbit IgG