CST, 12585S, CXXC1 Antibody

Polyclonal Antibody for studying CXXC1. Validated for Western Blotting. Highly specific and rigorously validated in-house, CXXC1 Antibody (CST #12585) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CXXC1 Antibody recognizes endogenous levels of total CXXC1 protein. Species Reactivity: Human, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp117 of human CXXC1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The CXXC finger protein 1 (CXXC1, CGBP, CFP1) is a key subunit of the human SET1 histone methyltransferase complex (1,2) that methylates histone H3 at Lys4 to create a mark of transcriptionally active promoters (3,4). CXXC1 is enriched at CpG islands where it selectively binds non-methylated CpG motifs to provide a link between global H3K4 methylation and CpG islands (5). Research studies have revealed a role for CXXC1 in the maintenance of cytosine methylation through direct interaction with DNMT1 (6-9). The epigenetic functions of CXXC1 are critical for normal embryonic development. Targeted deletion of the murine gene results in early embryonic lethality while Cxxc1-null embryonic stem (ES) cells exhibit increased apoptosis and fail to undergo differentiation following withdrawal of leukemia inhibitory factor LIF (6). Alternate Names 2410002I16Rik; 5830420C16Rik; CFP1; CGBP; CpG binding protein; CpG-binding protein; CXXC finger 1 (PHD domain); CXXC finger protein 1; CXXC-type zinc finger protein 1; CXXC1; DNA-binding protein with PHD finger and CXXC domain; hCGBP; HsT2645; PCCX1; PHD finger and CXXC domain-containing protein 1; PHF18; SPP1; ZCGPC1; zinc finger, CpG binding-type containing 1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 82 SOURCE: Rabbit