CST, 12619S, CTR9 (D1Z4F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SH2BP1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CTR9 (D1Z4F) Rabbit Monoclonal Antibody (CST #12619) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CTR9 (D1Z4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CTR9 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CTR9 protein. Background The PAF (RNA polymerase II (RNAPII) associated factor) complex was initially identified in yeast and is comprised of subunits PAF1, Leo1, Ctr9, Cdc73, RTF1 and Ski8 (1,2). The PAF complex plays an important role in transcription initiation and elongation by RNAPII by regulating the establishment of proper histone modifications such as histone H2B ubiquitination and the recruitment of the histone chaperone FACT (facilitates chromatin transcription) (3-5). The PAF complex also plays a role in mRNA processing and maturation by interacting with and recruiting the cleavage and polyadenylation specificity factor and cleavage stimulation factor complexes via the Cdc73 subunit (6,7). In addition, the Ski8 subunit of the PAF complex is part of the hSKi complex that regulates RNA surveillance, suggesting an important function of the complex in coordinating events associated with proper RNA maturation during transcription (1,5). Alternate Names CTR9; CTR9 homolog, Paf1/RNA polymerase II complex component; Ctr9, Paf1/RNA polymerase II complex component, homolog; Ctr9, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae); KIAA0155; p150; p150TSP; RNA polymerase-associated protein CTR9 homolog; SH2 domain binding protein 1 (tetratricopeptide repeat containing); SH2 domain-binding protein 1; SH2BP1; TPR-containing, SH2-binding phosphoprotein; TSBP Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140 Source/Isotype: Rabbit IgG