CST, 12636S, RIP4 Antibody

Polyclonal Antibody for studying ANKRD3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, RIP4 Antibody (CST #12636) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity RIP4 Antibody recognizes endogenous levels of total RIP4 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RIP4 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The receptor-interacting protein (RIP) family of serine-threonine kinases (RIP, RIP2, RIP3, and RIP4) are important regulators of cellular stress that trigger pro-survival and inflammatory responses through the activation of NF-κB, as well as pro-apoptotic pathways (1). In addition to the kinase domain, RIP contains a death domain responsible for interaction with the death domain receptor Fas and recruitment to TNF-R1 through interaction with TRADD (2,3). RIP-deficient cells show a failure in TNF-mediated NF-κB activation, making the cells more sensitive to apoptosis (4,5). RIP also interacts with TNF-receptor-associated factors (TRAFs) and can recruit IKKs to the TNF-R1 signaling complex via interaction with NEMO, leading to IκB phosphorylation and degradation (6,7). Overexpression of RIP induces both NF-κB activation and apoptosis (2,3). Caspase-8-dependent cleavage of the RIP death domain can trigger the apoptotic activity of RIP (8). Receptor-interacting serine-threonine kinase 4 (RIP4, ANKRD3, DIK, PKK, or RIPK4) is a membrane-associated, ankyrin repeat-containing member of the RIP family first identified in HaCat cells (9,10). RIP4 has been shown to be involved in keratinocyte differentiation as well as wound repair (11-13). Studies indicate that siRNA knockdown of RIP4 in human xenografted tumor cells suppresses Wnt-dependent growth while over-expression of RIP4 stabilized β-catenin and lead to an increase in Wnt-dependent gene expression (14). Alternate Names ANKK2; ANKRD3; ankyrin repeat domain 3; Ankyrin repeat domain-containing protein 3; CHANDS; DIK; MGC129992; MGC129993; NKRD3; PKC-delta-interacting protein kinase; PKK; PPS2; protein kinase C-associated kinase; receptor interacting serine/threonine kinase 4; receptor-interacting serine-threonine kinase 4; Receptor-interacting serine/threonine-protein kinase 4; RIP4; RIPK4; serine/threonine-protein kinase ANKRD3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 86 SOURCE: Rabbit