CST, 12658S, VEGF Receptor 2 (D5B1) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)

Monoclonal Antibody for studying VEGFR2. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, VEGF Receptor 2 (D5B1) Rabbit Monoclonal Antibody (Alexa Fluor^® 647 Conjugate) (CST #12658) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity VEGF Receptor 2 (D5B1) Rabbit Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant protein containing the carboxy-terminal 150 amino acid residues of human VEGF receptor 2 protein. Background Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951/996) and in the tyrosine kinase catalytic domain (Tyr1054/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells and angiogenesis (6-8). Alternate Names CD309; Fetal liver kinase 1; fetal liver kinase-1; FLK-1; FLK1; KDR; Kinase insert domain receptor; kinase insert domain receptor (a type III receptor tyrosine kinase); Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; tyrosine kinase growth factor receptor; Vascular endothelial growth factor receptor 2; VEGFR; VEGFR-2; VEGFR2; VGFR2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG