CST, 12660S, CABIN1 (D2B9F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CABIN1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CABIN1 (D2B9F) Rabbit Monoclonal Antibody (CST #12660) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CABIN1 (D2B9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total CABIN1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CABIN1 protein. Background Calcineurin binding protein CABIN1 was originally identified as an inhibitor of the calcium-dependent serine/threonine phosphatase, calcineurin. CABIN1 inhibits calcineurin signaling in T cells, regulating T cell receptor (TCR) signaling, transcription, and apoptosis (1-4). CABIN1 represses myocyte enhancer factor 2 (MEF2)-mediated transcription by recruiting chromatin remodeling enzymes (5), and also negatively regulates the activity of the tumor suppressor p53 (6). In response to genotoxic stress, CABIN1 is degraded and releases its inhibition of p53, allowing p53 to elicit cellular stress responses (7). CABIN1 is also involved in regulation of chromatin structure as part of the highly conserved HIRA/UBN1/CABIN1/ASF1A (HUCA) histone chaperone complex (8,9). Alternate Names CABIN; CABIN1; CAIN; calcineurin binding protein 1; calcineurin binding protein cabin 1; Calcineurin inhibitor; Calcineurin-binding protein cabin-1; KB-318B8.7; KIAA0330; PPP3IN Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 230 Source/Isotype: Rabbit IgG