CST, 12692S, SignalStain® Apoptosis (Cleaved Caspase-3) IHC Detection Kit

IHC Kit for studying Casp3 (Asp175) cleaved in the research area. Protocol Available protocols: Immunohistochemistry (Paraffin) Specificity / Sensitivity SignalStain Species Reactivity: Human, Mouse Source / Purification Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein. Rabbit (DA1E) mAb IgG XP SignalStain Isotype Control is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies. Background Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). Alternate Names Apopain; CASP-3; CASP3; Caspase-3; Caspase3; CPP-32; CPP32; SCA-1; Yama