CST, 12735T, NXF1 (D5X4G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying NXF1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, NXF1 (D5X4G) Rabbit Monoclonal Antibody (CST #12735) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity NXF1 (D5X4G) Rabbit Monoclonal Antibody recognizes endogenous levels of total NXF1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln211 of human NXF1 protein. Background mRNA export is a process that is tightly coupled to mRNA splicing (1-4). Splicing and packaging of mRNAs in the form of an mRNA-protein complex (mRNP) leads to the recruitment of the mRNA export adaptor THOC4/ALY, via its interaction with the splicing factor UAP56, forming a large complex termed the transcription-export complex (TREX) (1,2,5). THOC4/ALY then directly interacts with NXF1/TAP, a part of the heterodimer that targets the mRNP to the nuclear pore complex, resulting in the shuttling of mRNP out of the nucleus and into the cytoplasm (1-3,6). Alternate Names DKFZp667O0311; MEX67; mRNA export factor TAP; Nuclear RNA export factor 1; NXF1; TAP; tip associating protein; Tip-associated protein; Tip-associating protein Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 70 Source/Isotype: Rabbit IgG