CST, 12861S, Prostatic Acid Phosphatase (D3Y5P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ACPP. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Prostatic Acid Phosphatase (D3Y5P) Rabbit Monoclonal Antibody (CST #12861) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 46134 . Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity Prostatic Acid Phosphatase (D3Y5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACPP protein. This antibody is predicted to detect both the cellular and secreted isoforms of ACPP. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant human full-length prostatic acid phosphatase. Background Prostatic Acid Phosphatase (ACPP or PAP) is a member of the histidine acid phosphatase family. It is a non-specific phosphatase that is capable of dephosphorylating tyrosine residues as well as phospholipids under mildly acidic conditions. ACPP has ecto-5'-nucleotidase activity in pain-sensing neurons where it converts AMP to adenosine, suppressing the pain response (1,2). ACPP occurs as two isoforms that are both heavily glycosylated. The secreted phosphatase (sPAP) is found predominantly in the prostate and seminal plasma, while the cellular isoform (cPAP) is broadly expressed at very low levels and is associated with the plasma and lysosomal membranes (3-5). Cellular PAP has been shown to dephosphorylate ErbB2 at various tyrosine residues effectively terminating signaling (6). Furthermore, the physical interaction between cPAP and ErbB2 appears to regulate androgen sensitivity in prostate cancer cells. Loss of cPAP in androgen-sensitive prostate cancer cells results in the development of a castration-resistant phenotype suggesting that ACPP plays a significant role in prostate cancer cell growth (7). ACPP is expressed in metastatic cells arising from prostate cancer - especially in prostate-derived bone metastasis - suggesting that it may be a relevant diagnostic indicator of prostate cancer re-emergence in bone (8). Alternate Names 5'-NT; 5'-nucleotidase; acid phosphatase 3; acid phosphatase, prostate; ACP-3; ACP3; ACPP; Ecto-5'-nucleotidase; PAP; PAPf39; PPAP; Prostatic acid phosphatase; prostatic acid phosphotase; Thiamine monophosphatase; TM-PAP; TMPase Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 50 Source/Isotype: Rabbit IgG