CST, 12959S, Cytochrome c (D18C7) Rabbit Monoclonal Antibody (HRP Conjugate)

Monoclonal Antibody for studying cytochrome C. Validated for Western Blotting. Highly specific and rigorously validated in-house, Cytochrome c (D18C7) Rabbit Monoclonal Antibody (HRP Conjugate) (CST #12959) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Cytochrome c (D18C7) Rabbit Monoclonal Antibody (HRP Conjugate) recognizes endogenous levels of total cytochrome c protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c protein. Background Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). Alternate Names CYC; CYCS; Cytochrome c; cytochrome c, somatic; HCS; THC4 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG