CST, 13069S, Ring1A (D2P4D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Ring1A. Validated for WB,IP,IF,C&R. Available in 2 sizes. Highly specific and rigorously validated in-house, Ring1A (D2P4D) Rabbit Monoclonal Antibody (CST #13069) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:800 CUT&RUN: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), CUT&RUN Specificity / Sensitivity Ring1A (D2P4D) Rabbit Monoclonal Antibody recognizes endogenous levels of total Ring1A protein. This antibody does not cross-react with Ring1B. Species Reactivity: Human, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro316 of human Ring1A protein. Background Ring1A plays a role in polycomb group (PcG) protein function. PcG proteins are critically involved in transcriptional repression of Hox genes during development (1,2). PcG proteins form two distinct complexes: EED-EZH2 and the PRC complex, which is composed of at least Bmi1 and Ring1A/Ring1B. The EZH2-containing complex is responsible for the methylation of H3K27, and the PRC complex ubiquitylates H2A. EZH2 methylation is required prior to PRC ubiquitylation, and both are essential for Hox gene repression (1). It has recently been shown that PcG proteins silence a group of developmentally important regulator genes, referred to as bivalent genes (3). This regulation may be responsible for the ability of stem cells to self renew or switch to differentiate into multipotent progenitors. Aberrant epigenetic silencing by PcG proteins is also thought to be important in tumorigenesis (4). Alternate Names E3 ubiquitin-protein ligase RING1; Polycomb complex protein RING1; Really interesting new gene 1 protein; RING finger protein 1; RING-type E3 ubiquitin transferase RING1; RING1; RING1A; RNF1 Specification REACTIVITY: H R SENSITIVITY: Endogenous MW (kDa): 54 Source/Isotype: Rabbit IgG