CST, 13223S, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying Stat4 (Tyr693) phosphate. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (PE Conjugate) (CST #13223) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr693 of human Stat4 protein. Background The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9). Alternate Names Signal transducer and activator of transcription 4; signal transducer and activator of transcription 4 variant 3; SLEB11; STAT4 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG