CST, 13231S, Phospho-alpha-E-Catenin (Ser655/Thr658) Antibody

Polyclonal Antibody for studying Catenin-alphaE (Ser655/Thr658) phosphate. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Phospho-alpha-E-Catenin (Ser655/Thr658) Antibody (CST #13231) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Phospho-alpha-E-Catenin (Ser655/Thr658) Antibody recognizes endogenous levels of α-E-catenin protein only when phosphorylated at Ser655 and Thr658. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser655/Thr658 of human α-E-catenin protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Adherens junctions are dynamic structures that form cell-cell contacts and are important in development, differentiation, tissue integrity, morphology and cell polarity. They are composed of the transmembrane proteins, cadherins, which bind cadherins on adjacent cells in a calcium-dependent manner. On the cytoplasmic side of adherens junctions, the classic model states that cadherins are linked to the cytoskeleton through β- and α-catenin. α-E-catenin is ubiquitously expressed, α-N-catenin is expressed in neuronal tissue, and α-T-catenin is primarily expressed in heart tissue. Research studies have demonstrated that loss of E-cadherin and α-E-catenin occurs during the progression of several human cancers, indicating that the breakdown of adherens junctions is important in cancer progression (reviewed in 1). Research studies also suggest that, rather than acting as a static link between cadherins and actin, α-catenin regulates actin dynamics directly, possibly by competing with the actin nucleating arp2/3 complex (2,3). α-catenin also plays a role in regulating β-catenin-dependent transcriptional activity, affecting differentiation and response to Wnt signaling. α-catenin binds to β-catenin in the nucleus, preventing it from regulating transcription, and levels of both proteins appear to be regulated via proteasome-dependent degradation (4). Phosphorylation of α-E-catenin at Ser655 and Thr658 are post-translational modifications identified in a number of mass spectrometry studies, using a variety of tissue and cell types of both mouse and human origin (5-8). Alternate Names Alpha E-catenin; alpha-catenin; alpha-E-catenin; alphaE-catenin; Cadherin-associated protein; cadherin-associated protein,102kDa; CAP102; catenin (cadherin-associated protein), alpha 1, 102kDa; catenin alpha 1; Catenin alpha-1; CTNA1; CTNNA1; epididymis secretory sperm binding protein; FLJ36832; FLJ52416; MDPT2; Renal carcinoma antigen NY-REN-13 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 SOURCE: Rabbit