CST, 13328S, Phospho-SHP-2 (Tyr580) (D66F10) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying SHP-2 (Tyr584) phosphate. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-SHP-2 (Tyr580) (D66F10) Rabbit Monoclonal Antibody (PE Conjugate) (CST #13328) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-SHP-2 (Tyr580) (D66F10) Rabbit Monoclonal Antibody (PE Conjugate) detects endogenous level of SHP-2 only when phosphorylated at Tyr580. Species Reactivity: Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr580 of human SHP-2 protein. Background SHP-2 (PTPN11) is a ubiquitously expressed, nonreceptor protein tyrosine phosphatase (PTP). It participates in signaling events downstream of receptors for growth factors, cytokines, hormones, antigens, and extracellular matrices in the control of cell growth, differentiation, migration, and death (1). Activation of SHP-2 and its association with Gab1 is critical for sustained Erk activation downstream of several growth factor receptors and cytokines (2). In addition to its role in Gab1-mediated Erk activation, SHP-2 attenuates EGF-dependent PI3 kinase activation by dephosphorylating Gab1 at p85 binding sites (3). SHP-2 becomes phosphorylated at Tyr542 and Tyr580 in its carboxy terminus in response to growth factor receptor activation (4). These phosphorylation events are thought to relieve basal inhibition and stimulate SHP-2 tyrosine phosphatase activity (5). Mutations in the corresponding gene result in a pair of clinically similar disorders (Noonan syndrome and LEOPARD syndrome) that may result from abnormal MAPK regulation (6). Alternate Names BPTP3; CFC; JMML; METCDS; MGC14433; NS1; protein tyrosine phosphatase non-receptor type 11; protein tyrosine phosphatase-2; protein tyrosine phosphatase, non-receptor type 11; Protein-tyrosine phosphatase 1D; Protein-tyrosine phosphatase 2C; PTN11; PTP-1D; PTP-2C; PTP2C; PTPN11; SH-PTP2; SH-PTP3; SHP-2; Shp2; SHPTP2; Tyrosine-protein phosphatase non-receptor type 11 Specification REACTIVITY: M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG