CST, 13483T, Sec31A (E1J5M) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Sec31A. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Sec31A (E1J5M) Rabbit Monoclonal Antibody (CST #13483) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Sec31A (E1J5M) Rabbit Monoclonal Antibody recognizes endogenous levels of total Sec31A protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser1080 of human Sec31A protein. Background The coat protein complex II (COPII) is composed of five cytosolic proteins and includes the Sec23/24 complex, the Sec13/31 complex, and Sar1. The COPII coat is located at the ER/Golgi interface and is involved in transport of newly synthesized proteins from the ER to the Golgi apparatus (1). COPII formation is initiated through the binding of the activated G protein, Sar1, to the Sec23/24 complex to form a pre-budding complex that directly binds target molecules (1-3). This pre-budding complex further recruits Sec13/31 to form mature COPII coat (4,5). The Sec31 subunit of COPII coat interacts with Sec13 at the ER exit and is required for both vesicle formation and ER-Golgi transport. Two isoforms of human Sec31 have been identified, Sec31A and Sec31B, which share a sequence homology of 47.3% (6-8). Sec31A is ubiquitously expressed in tissues and organs, whereas Sec31B is enriched in brain and testis (7,8). In classical Hodgkin lymphoma, a novel fusion of Jak2 with Sec31A renders Jak2 constitutively active and subject to Jak2 inhibitor effects (9). Alternate Names ABP125; ABP130; DKFZp686N07171; HSPC275; HSPC334; KIAA0905; MGC90305; NEDSOSB; Protein transport protein Sec31A; protein-transport protein SEC31; SC31A; SEC31 homolog A (S. cerevisiae); SEC31 homolog A, COPII coat complex component; SEC31 homolog A, COPII coating complex component; SEC31-like 1; SEC31-like protein 1; SEC31-related protein A; SEC31A; SEC31L1; Web1-like protein; yeast Sec31p homolog Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95-140 Source/Isotype: Rabbit IgG