CST, 13511T, PKG-1 alpha (D10G2) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PKG1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PKG-1 alpha (D10G2) Rabbit Monoclonal Antibody (CST #13511) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PKG-1 alpha (D10G2) Rabbit Monoclonal Antibody recognizes endogenous levels of total PKG-1α protein. This antibody does not cross-react with PKG-1β. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human PKG-1α protein. Background Cyclic GMP-dependent kinases (cGK/PKG) belong to the AGC family of serine/threonine protein kinases. In mammals, two genes encode PKG-1 and PKG-2. Alternative PKG-1 splicing yields α and β isoforms, which display tissue-specific expression patterns in humans (1). All PKG family members are activated by increased cellular cGMP, which binds to the enzyme's regulatory domain inducing a conformational change and leading to enzyme activation. cGMP levels are increased through the activation of guanylyl cyclases, a process known to occur in part through nitric oxide (NO) signaling (2). In addition to well established roles in platelet activation and smooth muscle relaxation (3), PKG signaling is important in many biological processes including cardiac contractility, axon guidance, bone growth, contraction of intestinal smooth muscle, and erectile dysfunction (4). Alternate Names AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; cGMP-dependent protein kinase 1; cGMP-dependent protein kinase I; DKFZp686K042; FLJ36117; KGP1; MGC71944; PGK; PKG; PKG1; PRKG1; PRKG1B; PRKGR1A; PRKGR1B; protein kinase cGMP-dependent 1; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 78 Source/Isotype: Rabbit IgG