CST, 13721S, CD13/APN (E1Y7U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CD13. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CD13/APN (E1Y7U) Rabbit Monoclonal Antibody (CST #13721) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CD13/APN (E1Y7U) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD13/APN protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg90 of human CD13/APN protein. Background Aminopeptidase N (APN, CD13) is a widely expressed, membrane-bound proteolytic enzyme that breaks down peptides during digestion, cleaves cell surface antigens during antigen presentation, and acts as a receptor for human viruses, including several coronaviruses. This multifunctional protein is implicated in the regulation of many biological processes, including angiogenesis, cell proliferation, cell migration, inflammation and immune response (1,2). APN was originally identified as the cell surface antigen CD13, which is expressed in myeloid lineage hematopoietic cells and myeloid leukemia (3). Identified substrates of aminopeptidase N include the angiotensin I-III peptide hormones, the opioid peptide met-enkephalin, and cytokines MCP-1 and MIP-1 (4). Abnormal APN protein expression is seen in various forms of cancer, with high APN expression associated with poor survival in colon cancer and non-small cell lung cancer and silenced APN expression related to poor prognosis in prostate cancer (5-7). Alternate Names alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; alanyl aminopeptidase, membrane; Aminopeptidase M; Aminopeptidase N; AMPN; ANPEP; AP-M; AP-N; APN; CD13; gp150; hAPN; LAP1; membrane alanyl aminopeptidase; Microsomal aminopeptidase; Myeloid plasma membrane glycoprotein CD13; P150; PEPN Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 140-160 Source/Isotype: Rabbit IgG