CST, 13760S, XRN2 (D6L5F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying XRN2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, XRN2 (D6L5F) Rabbit Monoclonal Antibody (CST #13760) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity XRN2 (D6L5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total XRN2 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human XRN2 protein. Background 5'-3' exoribonuclease 2 (XRN2) is a nuclear exonuclease that degrades RNA containing a 5'-monophosphate to component mononucleotides. XRN2 also plays an important role in the termination of transcription at the 3'-end of genes by displacing RNA polymerase II (RNAPII) from the DNA strand (1,2). According to the ‘torpedo' model of transcription termination, XRN2 gains access to the 5' phosphate of the nascent RNA during co-transcriptional polyadenylation site cleavage. XRN2 degrades RNA at a faster rate than RNAPII-mediated RNA synthesis, resulting in the eviction of RNAPII from the template (3-5). In addition, XRN2 is essential for maturation of 5.8S and 28S ribosomal RNA and small nucleolar RNA molecules (2). Several research studies suggest that XRN2 plays a role in the quality control check of RNA molecules. XRN2 co-transcriptionally degrades aberrant nuclear mRNA transcripts that result from defective 5'mRNA capping, splicing, or 3'end formation (6). XRN2 exonuclease rapidly degrades hypomodified tRNA and excess miRNA molecules, indicating that XRN2 likely regulates tRNA and miRNA quality control as well (7-9). Alternate Names 5'-3' exoribonuclease 2; DHM1-like protein; Dhm1-like protein (mouse homolog); DHP protein; XRN2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG