CST, 13789S, UFD1 Antibody

Polyclonal Antibody for studying UFD1L. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, UFD1 Antibody (CST #13789) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UFD1 Antibody recognizes endogenous levels of total UFD1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UFD1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The ubiquitin fusion degradation 1 (UFD1) adaptor protein is a component of a protein complex essential for degradation of misfolded proteins by the endoplasmic reticulum-associated protein degradation (ERAD) pathway (1). The UFD1 protein contains a pair of conserved, amino-terminal ubiquitin-binding sites responsible for binding mono- and polyubiquitin molecules (2,3). The carboxy-terminal region of UFD1 contains binding sites for both the adapter protein NPL4 and the AAA ATPase VCP (4). The UFD1-NPL4 heterodimer binds VCP to create a protein complex responsible for export of misfolded proteins from the ER to the cytoplasm for ubiquitin-mediated degradation (5-7). The same protein complex may also be involved in disassembly of the spindle apparatus following mitosis (8). Alternate Names UB fusion protein 1; ubiquitin fusion degradation 1 like; ubiquitin fusion degradation 1 like (yeast); ubiquitin fusion degradation 1-like; Ubiquitin fusion degradation protein 1; Ubiquitin fusion degradation protein 1 homolog; ubiquitin recognition factor in ER associated degradation 1; Ubiquitin recognition factor in ER-associated degradation protein 1; UFD1; UFD1L Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit