CST, 13811S, Toll-like Receptor 5 (D9O8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TLR5. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Toll-like Receptor 5 (D9O8Q) Rabbit Monoclonal Antibody (CST #13811) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Toll-like Receptor 5 (D9O8Q) Rabbit Monoclonal Antibody recognizes transfected levels of total TLR5 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human TLR5 protein. Background Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in , play a pivotal role in innate immune responses (1-4). TLRs recognize conserved motifs found in various pathogens and mediate defense responses (5-7). Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes (4). The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain (1). Upon activation, TLRs associate with a number of cytoplasmic adapter proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adapter-like/TIR-associated protein (MAL/TIRAP), TIR domain-containing adapter-inducing IFN-β (TRIF), and Toll-receptor-associated molecule (TRAM) (8-10). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK (8,11-14). Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm. Toll-like receptor 5 (TLR5) is a pattern recognition receptor (PRR) that specifically recognizes bacterial flagellin (15). TLR5 binds flagellin directly on its lateral surface in a symmetrical arrangement and forms a 2/2 complex (16). TLR5 is expressed in epithelial cells, monocytes, and dendritic cells (DCs). TLR5 is an essential component of intestinal immunity at the mucosal barrier. It mediates immune tolerance to symbiotic flagellin during homeostasis and can lead to the production of chemokines and inflammatory cytokines when homeostasis is broken (17). The TLR5-microbiota axis is being investigated for prognostic and treatment potential of inflammatory bowel disease, and TLR5 agonists are being investigated in combination with immune checkpoint therapy as a strategy to treat previously unresponsive cancer patients (17,18). Alternate Names FLJ10052; MELIOS; MGC126430; MGC126431; SLE1; SLEB1; systemic lupus erythematosus susceptibility 1; TIL3; TLR5; toll like receptor 5; Toll-like receptor 5; Toll/interleukin-1 receptor-like protein 3 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 105 Source/Isotype: Rabbit IgG