CST, 14288S, PATL1/PAT1b (D8P1B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying PATL1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, PATL1/PAT1b (D8P1B) Rabbit Monoclonal Antibody (CST #14288) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity PATL1/PAT1b (D8P1B) Rabbit Monoclonal Antibody recognizes endogenous levels of total PATL1/PAT1b protein. This antibody does not cross-react with PATL2. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr432 of human PATL1/PAT1b protein. Background PATL1/PAT1b is the human homolog of the evolutionarily conserved Pat1/Mrt1 protein, which was first identified in (1,2). This protein is a critical component of the RNA decay machinery in the cytoplasmic processing bodies (P-bodies), localized foci of mRNA silencing and degradation (3). PATL1/PAT1b interacts with many key components of the RNA decay machinery. These include the 5' decapping proteins DDX6/RCK, DCP1, DCP2, EDC4, and the LSm1-7 ring (3,4) as well as the 3' deadenylation complex of CAF1-CCR4-NOT1 (3). Alternate Names FLJ36874; hPat1b; MGC125671; MGC125672; PAT1 homolog 1, processing body mRNA decay factor; PAT1-like protein 1; Pat1b; PATL1; protein associated with topoisomerase II homolog 1; protein associated with topoisomerase II homolog 1 (yeast); Protein PAT1 homolog 1; Protein PAT1 homolog b Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG