CST, 14300T, Miz-1 (D7E8B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ZBTB17. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Miz-1 (D7E8B) Rabbit Monoclonal Antibody (CST #14300) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Miz-1 (D7E8B) Rabbit Monoclonal Antibody recognizes endogenous levels of total Miz-1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro161 of human Miz-1 protein. Background Miz-1 (Zbtb17) is a poxvirus and zinc finger (POZ) transcription factor with an amino-terminal BTB/POZ domain and 13 carboxy-terminal zinc finger domains. Miz-1 plays a key role in cell cycle control through activation of the cyclin-dependent kinase inhibitors p15, INK4B, and p21 Waf1/Cip1 (1-4). The transcriptional activity of Miz-1 is repressed through direct interaction with Myc (1-4). In the presence of DNA damage, Myc is recruited to the p21 Waf1/Cip1 promoter by Miz-1 and blocks p53-mediated induction of p21 Waf1/Cip1, ultimately resulting in p53-mediated apoptosis rather than cell cycle arrest (4). Miz-1 also plays a role during lymphocyte development. In developing B and T cells, Miz-1 represses suppressor of cytokine signaling 1 (SOCS1) expression, which enables signaling through the IL-7 receptor and upregulation of the pro-survival protein Bcl-2 (5,6). Alternate Names Miz-1; MIZ1; Myc-interacting zinc finger protein 1; Myc-interacting Zn finger protein-1; pHZ-67; ZBT17; ZBTB17; zinc finger and BTB domain containing 17; Zinc finger and BTB domain-containing protein 17; Zinc finger protein 151; zinc finger protein 151 (pHZ-67); Zinc finger protein 60; ZNF151; ZNF60 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95 Source/Isotype: Rabbit IgG