CST, 14364S, IGF-II Receptor/CI-M6PR (D3V8C) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IGF2R. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, IGF-II Receptor/CI-M6PR (D3V8C) Rabbit Monoclonal Antibody (CST #14364) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 33388 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity IGF-II Receptor/CI-M6PR (D3V8C) Rabbit mAb recognizes endogenous levels of total IGF-II Receptor/CI-M6PR protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1675 of human IGF-II Receptor/CI-M6PR protein. Background Insulin-like growth factor II (IGF-II) receptor, also widely known as cation-independent mannose 6-phosphate receptor (CI-M6PR), is a multifunctional type I transmembrane glycoprotein that participates in the internalization of mannose-6-phosphate modified hydrolases and IGF-II from the plasma membrane (1,2). In the absence of ligands, IGF-II receptor is constitutively endocytosed from the cell surface to accumulate in the Golgi apparatus (3). In the presence of ligands, the receptor transports the mannose-6-phosphate modified hydrolases to acidified endosomes and lysosomes (4). The ligand-free receptor is then transported back to the Golgi compartment or the cell surface (4). In several research studies, IGF-II receptor has been recognized as a tumor suppressor in a number of cancers (5-7). Alternate Names 300 kDa mannose 6-phosphate receptor; cation-independent mannose-6 phosphate receptor; Cation-independent mannose-6-phosphate receptor; CD222; CI Man-6-P receptor; CI-M6PR; CI-MPR; CIMPR; IGF-II receptor; IGF2R; insulin like growth factor 2 receptor; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; M6P-R; M6P/IGF2 receptor; M6P/IGF2R; M6PR; MPR 300; MPR1; MPR300; MPRI Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 275 Source/Isotype: Rabbit IgG