CST, 14457T, ELMO1 (D4K2E) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ELMO1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ELMO1 (D4K2E) Rabbit Monoclonal Antibody (CST #14457) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ELMO1 (D4K2E) Rabbit Monoclonal Antibody recognizes endogenous levels of total ELMO1 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala567 of human ELMO1 protein. Background Engulfment and cell motility 1 (ELMO1) is a cell motility and migration protein that interacts with DOCK180 to form an atypical, two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). The resultant localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes including phagocytosis and cell migration (2-4). Research studies indicate that DOCK180 and ELMO1 regulate cell migration in lymphocytes (5) and in ovarian cancer cells (6). ELMO1 also promotes Rac1-dependent cell motility through its interaction with the adaptor protein Nck-1 (7), and binds Arhgef16 to promote RhoG/Rac1-dependent engulfment of apoptotic cells by phagocytes (8). Polymorphisms in the corresponding gene may be associated with susceptibility to diabetic neuropathy seen in selected populations of type II diabetic patients (9,10). Alternate Names CED-12; ced-12 homolog 1; CED12; ELMO-1; ELMO1; engulfment and cell motility 1; Engulfment and cell motility protein 1; KIAA0281; MGC126406; Protein ced-12 homolog Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 80 Source/Isotype: Rabbit IgG