CST, 14461S, Phospho-PLC gamma1 (Tyr783) (D6M9S) Rabbit Monoclonal Antibody (PE Conjugate)

Monoclonal Antibody for studying PLCg1 (Tyr783) phosphate. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Phospho-PLC gamma1 (Tyr783) (D6M9S) Rabbit Monoclonal Antibody (PE Conjugate) (CST #14461) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Phospho-PLC gamma1 (Tyr783) (D6M9S) Rabbit Monoclonal Antibody (PE Conjugate) recognizes endogenous levels of PLCγ1 protein only when phosphorylated at Tyr783. Species Reactivity: Human, Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr783 of human PLCγ1 protein. Background Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli, such as hormones, growth factors, and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP ) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP ) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ, and PLCε. Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (2). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (3). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (4). PLCγ2 is engaged in antigen-dependent signaling in B cells and collagen-dependent signaling in platelets. Phosphorylation by Btk or Lck at Tyr753, 759, 1197, and 1217 is correlated with PLCγ2 activity (5,6). Alternate Names 1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; NCKAP3; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C gamma 1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; phospholipase C, gamma 1; phospholipase C, gamma 1 (formerly subtype 148); PLC-148; PLC-gamma-1; PLC-II; PLC1; PLC148; PLCG1; PLCgamma1; triphosphoinositide phosphodiesterase Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG