CST, 14614S, HTATIP2/TIP30 Antibody

Polyclonal Antibody for studying HTATIP2. Validated for Western Blotting. Highly specific and rigorously validated in-house, HTATIP2/TIP30 Antibody (CST #14614) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HTATIP2/TIP30 Antibody recognizes endogenous levels of total HTATIP2 protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His231 of human HTATIP2 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background The HIV-1 Tat interactive protein 2 (HTATIP2, TIP30, CC3) is an oxidoreductase that was originally identified as a metastatic tumor suppressor and Tat-mediated proapoptotic gene transcription cofactor (1,2). HTATIP2 protein contains a short-chain dehydrogenase (SDR) domain and a NADPH binding motif important for HTATIP2 interaction with importins and inhibition of nucleocytoplasmic transport (3,4). Research studies demonstrate that induced overexpression of HTATIP2 predisposes cells to apoptosis by inhibiting the nuclear transport of important signaling proteins (e.g. p53, activated notch1) and several key targets of the DNA repair process (5-7). HTATIP2 is part of a protein complex, with Rab5a, endophilin B1, and ACSL4, that may regulate EGFR receptor endosomal trafficking, degradation, and cytoplasmic/nuclear signaling (8,9). Silencing of HTATIP2 promotes tumor cell survival under low glucose conditions by inducing increased expression of mitochondrial respiratory proteins and glucose metabolic enzymes (10). Alternate Names 30 kDa HIV-1 TAT-interacting protein; CC3; FLJ26963; HIV-1 Tat interactive protein 2; HIV-1 Tat interactive protein 2, 30kDa; HIV-1 TAT-interactive protein 2; HTAI2; HTATIP2; Oxidoreductase HTATIP2; SDR44U1; short chain dehydrogenase/reductase family 44U, member 1; Tat-interacting protein (30kD); TIP30 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 28, 32 SOURCE: Rabbit