CST, 14625S, ING1b (D3D5Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ING1b. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ING1b (D3D5Z) Rabbit Monoclonal Antibody (CST #14625) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ING1b (D3D5Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total ING1b protein. This antibody specifically recognizes the ING1b p33 isoform (UniProt# Q9UK53-2). Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ING1b protein. Background Inhibitor of growth 1 (ING1) is a member of an evolutionarily conserved family of tumor suppressor proteins and transcription regulators (1,2). Differential mRNA splicing generates several ING1 isoforms, with the widely expressed ING1b (p33) isoform playing key roles in cell cycle regulation, apoptosis, and senescence (2-5). All ING family proteins contain a plant homeodomain (PHD) that is thought to recognize and bind methylated lysine residues on histone proteins (6,7). The ING1 protein regulates expression of genes through its association with histone acetyltransferase and deacetylase complexes. The PHD of ING1 may facilitate the recruitment of these chromatin-modifying enzymes to targets genes that are regulated by various transcription factors, such as p53 (2, 8-10). Consistent with its role as a tumor suppressor, alterations in ING1 expression levels and cytoplasm localization have been observed in several cancers but mutations in the corresponding gene in cancers are uncommon (11-14). Alternate Names growth inhibitor ING1; growth inhibitory protein ING1; ING1; ING1 iso2; inhibitor of growth family member 1; inhibitor of growth family, member 1; Inhibitor of growth protein 1; p24ING1c; p33; p33ING1; p33ING1b; p47; p47ING1a; tumor suppressor ING1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 33 Source/Isotype: Rabbit IgG