CST, 14657T, FANCA (D1L2Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying FancA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, FANCA (D1L2Z) Rabbit Monoclonal Antibody (CST #14657) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity FANCA (D1L2Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total FANCA protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala514 of human FANCA protein. Background Fanconi anemia (FA) is an autosomal recessive genetic disorder that results in chromosomal breakage, bone marrow failure, hypersensitivity to DNA cross-linking agents (such as mitomycin C), and a predisposition to cancer (1). The ubiquitously expressed FA complementation group A protein (FANCA, FAA) is a component of the FA nuclear complex that also contains FANCB, FANCC, FANCE, FANCF, FANCG, FANCL, and FANCM. In response to DNA damage, the FA nuclear complex induces mono-ubiquitination of FANCD2 and FANCI (2). FANCJ/BRIP1, FANCD1/BRCA2 and FANCN/PALB2 are then recruited to sites of DNA damage along with other DNA repair proteins. FA signaling is important in maintenance of chromosome stability and control of mitosis (3). DNA-damage-dependent localization and stability of FANCA protein regulates FA complex function and localization. Interaction between FANCA protein and the Hsp90 chaperone protein regulates FANCA protein stability and turnover, and may play a role in controlling the FA DNA damage pathway (4). Mutations in the corresponding gene are responsible for the majority of cases of Fanconi anemia (5). Alternate Names FA; FA complementation group A; FA-H; FA1; FAA; FACA; FAH; FANCA; FANCH; Fanconi anemia complementation group A; Fanconi anemia group A protein; Fanconi anemia, complementation group A; Fanconi anemia, complementation group H; Fanconi anemia, type 1; MGC75158; Protein FACA Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG