CST, 14667S, ACD (D4E2R) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TPP1/ACD. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, ACD (D4E2R) Rabbit Monoclonal Antibody (CST #14667) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity ACD (D4E2R) Rabbit Monoclonal Antibody recognizes endogenous levels of total ACD protein. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu169 of human ACD protein. Background TPP1 is encoded by the the gene, and is one of six core proteins of the shelterin complex (TRF1, TRF2, Rap1, TIN2, POT1 and TPP1) that regulates telomere length and integrity. This nuclear protein complex localizes to telomeres, and protects the natural ends of chromosomes from inappropriate processing by DNA repair pathways (1). TPP1 was identified in screens for proteins that bind TIN2, which is considered to be the central component of the shelterin complex (1). TPP1 contains two protein-protein interaction domains that facilitate shelterin complex function: a carboxy-terminal TIN2-binding domain and a more central POT1-binding domain. Heterodimerization of TPP1 with POT1 promotes binding to single-stranded telomeric DNA, which facilitates telomere elongation and protection by the shelterin complex. The TPP1 protein also contains a TEL patch, a collection of surface amino acids that recruits telomerase and modulates its processivity (2). In addition to playing an important role in normal development (3), TPP1 is implicated in the etiology of selected diseases. For example, mutations in that alter the composition of the TEL patch have been linked to Hoyeraal-Hreidarsson syndrome, a clinically severe form of dyskeratosis congenita characterized by hematopoietic stem cell dysfunction, bone marrow failure, and a predisposition to cancer (4,5). Alternate Names ACD; ACD shelterin complex subunit and telomerase recruitment factor; adrenocortical dysplasia homolog; adrenocortical dysplasia homolog (mouse); Adrenocortical dysplasia protein homolog; PIP1; POT1 and TIN2 organizing protein; POT1 and TIN2-interacting protein; PTOP; TIN2 interacting protein 1; TINT1; TPP1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 58 Source/Isotype: Rabbit IgG