CST, 14684T, Na Channel beta1 Subunit (D9T5B) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SCN1B. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Na Channel beta1 Subunit (D9T5B) Rabbit Monoclonal Antibody (CST #14684) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Na Channel beta1 Subunit (D9T5B) Rabbit Monoclonal Antibody recognizes endogenous levels of total sodium channel β1 subunit protein. This antibody also cross-reacts with an unidentified protein of 60 kDa in whole brain lysate. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala51 of human sodium channel β1 subunit protein. Background Mammalian voltage-gated sodium channels (VGSCs) are composed of a pore-forming α subunit and one or more regulatory β subunits (1). Four separate genes (SCN1B-SCN4B) encode the five mammalian β subunits β1, β1B, β2, β3, and β4. In general, β subunit proteins are type I transmembrane proteins, with the exception of secreted β1B protein (reviewed in 2). β subunits regulate α subunit gating and kinetics, which controls cell excitability (3,4). Sodium channel β subunits also function as Ig superfamily cell adhesion molecules that regulate cell adhesion and migration (5,6). Additional research reveals sequential processing of β subunit proteins by β-secretase (BACE1) and γ secretase, resulting in ectodomain shedding of β subunit and generation of an intracellular carboxy-terminal fragment (CTF). Generation of the CTF is thought to play a role in cell adhesion and migration (7,8). Multiple studies demonstrate a link between β subunit gene mutations and a number of disorders, including epilepsy, cardiac arrhythmia, multiple sclerosis, neuropsychiatric disorders, neuropathy, inflammatory pain, and cancer (9-13). The sodium channel β1 subunit (SCN1B) plays a crucial role in neuronal migration and pathfinding during brain development (14). Mutations in the corresponding gene are associated with generalized epilepsy with febrile seizures plus 1 (15), Brugada syndrome (16), and familial atrial fibrillation (17). A loss of function mutation results in a severe form of pediatric epileptic encephalopathy known as Dravet syndrome (18). Alternate Names ATFB13; BRGDA5; EIEE52; GEFSP1; SCN1B; sodium channel beta-1 subunit; Sodium channel subunit beta-1; sodium channel, voltage gated, type I beta subunit; sodium channel, voltage-gated, type I, beta; sodium voltage-gated channel beta subunit 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38 Source/Isotype: Rabbit IgG