CST, 14689S, MLL1 (D2M7U) Rabbit Monoclonal Antibody (Amino-terminal Antigen)

Monoclonal Antibody for studying MLL. Validated for WB,IP,C&R,C&T. Available in 2 sizes. Highly specific and rigorously validated in-house, MLL1 (D2M7U) Rabbit Monoclonal Antibody (Amino-terminal Antigen) (CST #14689) is ready to ship. Product Usage Information The CUT&RUN dilution was determined using CUT&RUN Assay Kit # 86652 . The CUT&Tag dilution was determined using CUT&Tag Assay Kit # 77552 . Western Blotting: 1:1000 Immunoprecipitation: 1:50 CUT&RUN: 1:50 CUT&Tag: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, CUT&RUN, CUT&Tag Specificity / Sensitivity MLL1 (D2M7U) Rabbit Monoclonal Antibody (Amino-terminal Antigen) recognizes endogenous levels of total MLL1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human MLL1 protein. Background The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1, and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). MLL1 functions as a master regulator of both embryogenesis and hematopoiesis, and is required for proper expression of Hox genes (7,8). MLL1 is a large, approximately 4000 amino acid, protein that is cleaved by the taspase 1 threonine endopeptidase to form N-terminal (MLL1-N) and C-terminal MLL1 (MLL1-C) fragments, both of which are subunits of the functional MLL1/COMPASS complex (9,10). MLL1-N, MLL1-C, WDR5, RBBP5, and ASH2L define the core catalytic component of the MLL1/COMPASS complex, which is recruited to target genes and methylates histone H3 lysine 4 to regulate transcriptional initiation (11). At least 60 different MLL1 translocation partners have been molecularly characterized and associated with various hematological malignancies. The most common translocation partners include AF4, AF9, ENL, AF10, ELL, and AF6 (8,12,13). With the exception of AF6, all of these partners are nuclear proteins that function to positively regulate transcriptional elongation. AF4, AF9, and ENL are all components of the super elongation complex (SEC), while AF4, AF9, AF10, and ENL all interact with the histone H3 lysine 79 methyltransferase DOT1L. Many MLL1 target genes are normally regulated by promoter-proximal pausing, with the release of RNA polymerase and transcriptional elongation occurring in response to proper stimuli (14). The association of MLL1 translocation partners with SEC and DOT1L suggest that MLL1-fusion proteins may function to sustain specific gene expression programs by constitutively activating transcriptional elongation. Alternate Names ALL-1; ALL1; C-terminal cleavage product of 180 kDa; CDK6/MLL fusion protein; CXXC-type zinc finger protein 7; CXXC7; FLJ11783; Histone-lysine N-methyltransferase 2A; Histone-lysine N-methyltransferase MLL; HRX; HTRX; HTRX1; KMT2A; lysine (K)-specific methyltransferase 2A; lysine methyltransferase 2A; Lysine N-methyltransferase 2A; mixed lineage leukemia 1; MLL; MLL cleavage product C180; MLL cleavage product N320; MLL-AF4 der(11) fusion partner; MLL/GAS7; MLL/GAS7 fusion partner; MLL/GMPS fusion partner; MLL1; MLL1A; Myeloid/lymphoid or mixed-lineage leukemia; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); Myeloid/lymphoid or mixed-lineage leukemia protein 1; N-terminal cleavage product of 320 kDa; p180; p320; TET1-MLL; Trithorax-like protein; TRX1; WDSTS; Zinc finger protein HRX Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 300 Source/Isotype: Rabbit IgG