CST, 14771T, CEACAM1 (D1P4T) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CEACAM1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CEACAM1 (D1P4T) Rabbit Monoclonal Antibody (CST #14771) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity CEACAM1 (D1P4T) Rabbit Monoclonal Antibody recognizes endogenous levels of total CEACAM1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala460 of human CEACAM1 protein. Background CEACAM1 (also known as C-CAM and CD66a) is a member of CEA-related cell-adhesion molecule (CEACAM) subfamily of the carcinoembryonic antigen (CEA) family (1). CEACAM1 is expressed by certain epithelial, endothelial, lymphoid, and myeloid cells. Human CEACAM1 has many different splice variants; the abundance of CEACAM1 and the relative ratio of the different isoforms varies markedly among cell types and may be regulated in a context-dependent fashion. The isoforms with long (L) and short (S) cytoplasmic tails have different signaling properties. Notably, L isoforms contain a functional ITIM (immunoreceptor tyrosine-based inhibitory motif) and several serine and threonine residues that could serve as potential phosphorylation targets. The extracellular domain of CEACAM1 is heavily glycosylated, making its apparent molecular weight during electrophoresis much larger than its predicted size (57.6 kDa) (2). CEACAM1 mediates intercellular adhesion through homo- and heterophilic interaction with other members of the CEACAM family. Studies indicate that CEACAM1 plays important roles in angiogenesis, neovascularization, insulin signaling, T cell signaling, and tumorigenesis (3-8). In addition, CEACAM1 can function as a receptor for several microbial pathogens (9,10). Alternate Names antigen CD66; BGP; BGP-1; BGP1; BGPI; Biliary glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen related cell adhesion molecule 1; Carcinoembryonic antigen-related cell adhesion molecule 1; carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein); CD66a; CD66a antigen; CEA cell adhesion molecule 1; CEACAM1; CEAM1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 160 Source/Isotype: Rabbit IgG